Russian scientists have developed a rapid test for chicken and pork in processed meat products, which takes 33 minutes to complete and can be done in-situ without the need of laboratory equipment. The test consists of a 3 min of crude DNA extraction, 20 min of recombinase polymerase amplification (RPA) at 39 °C, and 10 min of lateral flow assay (LFA) detection. The RPA–LFA assay is based on designed fluorescein and biotin-labelled primers developed from the cytochrome B gene, which can identify as little as 0.001% w/w  of the target meat component. The assay was tested on  processed meat products and to meat after heating, and the results were confirmed by real-time PCR.


continue reading

E-mail me when people leave their comments –

You need to be a member of safefood Knowledge Network to add comments!

Join safefood Knowledge Network