During 2017–2019, a comprehensive set of archived serum samples from 22,940 market-weight pigs from 25 slaughterhouses in 10 US states was collected for an unrelated prevalence study of Toxoplasma and Trichinella. The samples were collected from slaughterhouses processing adult market-weight pigs >6 months of age: ≈250-pound market-weight hogs 6 months of age and female pigs >1 year of age. The blood samples were collected on the kill floor at the slaughterhouses, and serum was separated and stored frozen (−20°C) at the US Department of Agriculture–Agricultural Research Service, Beltsville Agricultural Research Center (Beltsville, MD, USA).

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